8/8/2023 0 Comments Waters acquity sample manager![]() ![]() Put your sample into the correct position in the UPLC Sample Manager and click “Finish” to complete the process. The mass options are optional and are useful especially if your sample does not absorb in UV.Ĭlick “Next” and the system displays a page telling you where to put your sample which will be either plate 1 or 2 of the Sample Manager. Here you can enter a description for your sample, so that you can later identify your spectra, and your name. Click "Next" to input some data about yorself and your sample. acidic and basic (The methods available will be updated to offer different gradients along with acidic and basic methods). Selecting more than one method will allow you to run different methods on the same sample vial location e.g. Select the method and hit the + icon to move your choice to the right hand side of the window. ![]() You will find a brief description of the method in the lower right of the window. Having selected your group select “Next” which will allow you to pick the method(s) you want to use. If your group is not listed please contact a member of the mass spec team for assistance. The OpenLynx window allows you to log your samples in to the system, choose a method and then inform you where to place your sample in the autosampler of the UPLC.Ĭlick on the button labelled “Login Samples…” A new window will appear where you can select your research group. ![]() When you first use the system you should see a desktop looking like this: High concentrations will saturate the UPLC column leading to poor chromatography which will in turn lead to bad results. Sample concentration should be no more than 0.1mg/ml. THIS WILL PREVENT SAMPLES CRASHING OUT OF SOLUTION AND BLOCKING TUBING/COLUMNS. SAMPLES MUST BE DISSOLED IN 95% Water / 5% ACETONITRILE OR AT LEAST 50% WATER / 50% ACETONITRILE. SAMPLE VIALS MUST INCLUDE A PRE-SLIT SEPTA. The Open Access UPLCMS system is a self-service system utilising a Waters H-Class UPLC with detection via a Waters PDA and a Waters SQD2 single quadrupole mass spectrometer.ĪLL SAMPLES MUST BE FILTERED DOWN TO 0.2 micron. ![]()
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